Immunologie Research en Kliniek

Immunology Research and Clinic

Studiewijzer 2021/2022

Globale inhoud

The lectures:

• Interactions between immunological systems (innate-acquired/immunological cells and soluble immunological factors)
• Cell biological processes in immunology (synapse formation, signal transduction, antigen presentation)
• Antibodies and autoimmunity (IgG4 versus IgG1, immune complexes, RA, therapeutic antibodies, B cell differentiation)
• Inflammation and complement activation (cell death and inflammation, complement regulation)
• Tumor immunology (dendritic cell therapy, adoptive T cell transfers, TLR agonists)
• Big data analyses in immunology

The seminars:

• FACS assignment based on the FACS-diva program
• The structure of proteins using the proximity plot
• Statistical assignment

The experiments:

• Purification of granulocytes followed by a phagocytosis experiment.
• Purification of monocytes by density separation followed by positive selection with antibody coupled magnetic beads.
• The differentiation of immature dendritic cells from the purified monocytes.
• Maturation of immature dendritic cells by cytokine cocktails or TLR-agonists.
• Measuring different cytokines with ELISA.
• Immunofluorescence of T lymphocytes.
• To visualize the immune synapses between a T-cell and a cancer cell by immunofluorescence.
• Determination of the activity of the classical and the alternative complement route in patient sera using hemolytic tests.
• Creating and presentation of a research proposal based on techniques and knowledge obtained in this course.

Studiemateriaal

Literatuur

• Janeway's Immunobiology, 9th edition, Kenneth M. Murphy ISBN 9780815345510

Practicummateriaal

• Laboratory coat is mandatory.

• Practical manual to be purchased at the start of the course (€10,-).

• Lab journal

Leerdoelen

• The student can explain the theory of main immunological interactions and cell biological process in immunology and discuss about their implications.
• The student can explain the theory of main processes and therapies in tumor immunology and discuss about their implications.
• The student can explain the role of antibodies in autoimmunity and discuss about its implications.
• The student can explain the processes involved in inflammation and discuss about its implications.
• The student can explain how big data in immunology can be analysed.
• The student is able to apply his or her own knowledge to solve structure-functional questions in immunology in computer practical.
• The student is able to apply the acquired knowledge to test immunological scientific questions in the practical.
• The student can analyse and interpret FACS and proximity plots.
• The student can independently perform humoral and cellular immunological techniques for the lab.
• The student can interpret the data and draw the right conclusions from the experiments.
• - The student can explain how the conclusions were reached.
• The student can argue why the conclusions are correct.
• The student can devise follow-up experiments on the basis of their own interpretation of the results of the experiments carried out.
• The student can conceive, plan, perform and present and experiment within the framework of the immunological subject provided.
• The student can succinctly and substantively display the execution, results and conclusions of the experiments in the lab journal

Onderwijsvormen

• Werkcollege
• Zelfstudie
• (Computer)practicum
• Hoorcollege

Verdeling leeractiviteiten

Aanwezigheid

Aanwezigheidseisen opleiding (OER-B):

• Deelname aan alle practica, computerpractica en werkcolleges in het curriculum is verplicht.
  

Aanvullende eisen voor dit vak:

• Participation in all practicals, computer practicals and lectures in the curriculum is mandatory.
• Presence at the lectures is strongly recommended.
• All practicals and seminars are compulsory. If you are unable to attend for one day, e.g. in case of illness, we will search for a solution together with you.
• Please report your absence to s.kruithof@sanquin.nl
• In case of unexpected absence, please send an e-mail to C.Kuijk@sanquin.nl (Carlijn Kuijk) or call 0205257772.
• If you have not been present please contact us to determine how to catch up on the missed practical.
• Make sure you own the missed lesson material on the day you come back. On this day you must also have prepared the practical.
• If you are not able to follow an education for a long period of time (longer than 1 week), please contact the study advisor.
• NB Covid-19: Keep to the RIVM guidelines at all times, even if this means missing one or more compulsory educational meetings. We will discuss together how we can try to solve problems.

Toetsing

Testing of the theory part takes place at the end of the course in the form of a theory exam (6 open questions with sub-questions, questions testing the ability to reflect learned knowledge and according to insight). This forms 40% of the final grade of the course. The theory exam can be retaken in the official retake period.

The practical exam forms the other 60% of the final grade. Participation in the practical is mandatory. During the practicals, the student's skills are assessed several times. See the syllabus. The practicals have an obligation to be present and a positive, active attitude of the student is expected. The practical assessment remains valid for one year. Students who have an insufficient assessment for the practicals can only retake the practicals in the next academic year.

An assessment of both parts of at least 5 is a prerequisite for the awarding of the final grade of the course. The student has passed the course if the final grade is 5.5 or higher.

Fraude en plagiaat

Dit vak hanteert de algemene 'Fraude- en plagiaatregeling' van de UvA. Hier wordt nauwkeurig op gecontroleerd. Bij verdenking van fraude of plagiaat wordt de examencommissie van de opleiding ingeschakeld. Zie de Fraude- en plagiaatregeling van de UvA: http://student.uva.nl

Weekplanning

Rooster

Het rooster van dit vak is in te zien op DataNose.

Aanvullende informatie

Vaccination against hepatitis B is mandatory. Students with serious philosophical objections are advised to report to the study advisor for a talk. The own contribution of the vaccination is € 55,- and has to be paid in advance by the student (this can be done at the education desk of the ESC at the Science Park).

Maximum 24 students.

Verwerking feedback studenten

We zijn heel blij met de zo positieve evaluatie van de studenten. 

Ik zal komend jaar in mijn inleidende colleges de rode draad van de hoorcolleges en practica nog duidelijker maken. Ik lees dat dat mogelijk goed is. Fijn te zien dat duidelijk opgepakt is door de studenten dat de inhoud van onze colleges zo opgesteld is dat het aansluit bij de practica onderdelen. 

1 studente had liever online onderwijs gehad. Ik ben verheugd dat de anderen blij waren dat we juist veel moeite hadden gedaan om zo veel mogelijk live te doen, binnen de beperkingen van de coronamaatregelen. Dit jaar gaan we naar aanleiding van de evaluatie van de studenten, onze eigen ervaringen en de situatie in de pandemie, terug naar live colleges op het Science Park omdat dit de interactie en kennis-uitwisseling bevordert.

Door de 1,5 meter-maatregelen hebben we soms in de practica studenten een onderdeel alleen laten doen in plaats van in een tweetal. Dit hebben we gedaan voor onderdelen die nauwelijks meer geld gingen kosten als men het individueel ging doen. We merkten al tijdens de practica dat veel studenten het heel fijn vonden soms alleen een onderdeel uit te kunnen voeren en vonden dat ze zo meer leerden. Dat gaan we blijvend erin houden.

Contactinformatie

Coördinator

• prof. dr. S.M. van Ham

Docenten

• Ing. Carlijn Kuijk
• Ing. Irma Rensink
• Prof. dr. Marieke van Ham
• Dr. Anja ten Brinke
• Dr. Sabrina Pollastro
• Dr. Gertjan Wolbink
• Dr. Theo Rispens