Separation Sciences

6 EC

Semester 1, period 1

52548SES6Y

Owner Master Chemistry (joint degree)
Coordinator prof. dr. Govert Somsen
Part of Master Chemistry (joint degree), track Analytical Sciences, Master Forensic Science, year 2

Course manual 2021/2022

Course content

The course comprises theory and practice of liquid and gas chromatography, and of capillary electrophoresis, including the common detection principles used with these separation techniques.

The fundamental principles of chromatography and capillary electrophoresis (e.g. retention, migration, dispersion, stationary-phase properties) and signal processing (e.g. statistical moments) will be addressed. The characteristics and differences of packed-column and open-tubular separations will be outlined. Attention will be paid to instrumental aspects, such as injection systems for gas chromatography.

For liquid chromatography, various phase systems including normal-phase, reversed-phase, and ion-exchange, will be discussed. The principles and application of gradient elution will be treated.

Other topics include an introduction to two-dimensional liquid and gas chromatography, sample preparation, and polymer separations.

As part of the course, students will give a short presention on a scientific paper on separations.

 

Study materials

Literature

  • will be available on Canvas

Other

  • Handouts of the lectures

    Exercises for problem solving

Objectives

  • Understand the principles and applications of the main separation techniques used in contemporary analytical science.
  • Master the theory and practice of modern liquid chromatography, gas chromatography and capillary electrophoresis.
  • Know and rationally use performance parameters of separation methods.
  • Read, understand, summarize and present recent papers on separation science.

Teaching methods

  • Lecture
  • Seminar
  • Presentation/symposium

Learning activities

Activity

Number of hours

Lectures/seminars

44

Presentation incl. preparation

12

Zelfstudie

120

 

Attendance

This programme does not have requirements concerning attendance (TER part B).

Additional requirements for this course:

The literature presentation is a requirement. Without literature presentation no grade will be provided.

Assessment

Item and weight Details

Final grade

0.9 (90%)

Tentamen

Must be ≥ 5, Mandatory

0.1 (10%)

Literature Presentation

Mandatory

The grade for the written exam should be at least 5.0.

Inspection of assessed work

Contact the course coordinator to make an appointment for inspection.

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

wk day date room lecturer topic    
               
36 Tue 7-Sep see DataNose or time schedule on Canvas BP Fundamentals of Chromatography
  Wed 8-sep   BP Dispersion  
  Fri 10-Sep   BP Peaks and Moments  
37 Tue 14-Sep   GS LC modes I  
  Fri 17-Sep   GS LC modes II  
38 Tue 21-Sep   BP Gradient Elution
  Wed 22-Sep   BP OTC  
  Fri 24-Sep   GS LC Detection  
39 Tue 28-Sep   BP Polymer Separations
  Fri 1-Oct   GS Sample Preparation
40 Tue 5-Oct   GS CE 1
  Wed 6-Oct   GS CE2
  Fri 8-Oct   GS CE detection/injection & MEKC
41 Tue 12-Oct   PS GC & injection
  Fri 15-Oct   PS 2D GC    
42 Tue 19-Oct     Student presentations
  Wed 20-Oct   BP/GS/PS Question hour
  Fri 22-Oct     Student presentations
43 Tue 26-Oct   -- Exam

Contact information

Coordinator

  • prof. dr. Govert Somsen

Docenten

Dr. Bob Pirok (BP)

Prof. Peter Schoenmakers (PS)

Prof. Govert Somsen (GS)