Course manual 2021/2022

Course content

The field of ecological genomics strives to uncover the genetic and molecular mechanisms influencing the response and adaptation of organisms to their natural environments. Achieving this requires a good understanding of ecologically important phenotypes and needs a species with a well-developed genomic tool-kit. Molecular biological and genomic tools have primarily been developed for mammalian and agricultural model organisms (such as yeast and Arabidopsis) representing a narrow spectrum of phenotypes, whereas many organisms that are the focus of ecological research have had limited genomic resources devoted to them.

In this course, you will learn how ecogenomics unites genomic and ecological approaches by exploring the natural variation in model or crop species and their relatives. We will explore the underlying principles of genomics and how they are being applied in ecogenomics. We will focus on ecogenomic studies of abiotic and biotic stress responses . Specific approaches that will be discussed and demonstrated include: genome sequencing, genetic mapping, expression analysis (transcriptomics), metabolomics and epigenomics. Specific analytical techniques that will be done “hands-on” will include: the use of genomic databases and tools, quantitative trait loci (QTL) analysis, stress physiology experiments, comparative sequence analysis, promoter analysis and genome-wide association mappping.

Study materials

Literature

  • Several textbooks will be used by the lecturers, including "Molecular Biology of the Cell" by Alberts et al, "Evolutionary Analysis" by Freeman & Herron, "A Primer of Genome Science" by Gibson and Muse. Other reading materials are provided on Canvas.

Syllabus

  • A practical manual will be provided through Canvas at the start of the course.

Other

  • Selected reviews and primary literature are available on Canvas.

Objectives

  • Explain the strategy of Ecogenomics in answering biological questions
  • Explain the principles of and difference between association mapping and mapping in biparental populations
  • Explain the principle of (epi-)genomics, transcriptomics, metabolomics
  • Explain how (epi-)genetic information translates into phenotypes
  • Analyse and criticize a scientific paper employing an ecogenomics approach
  • Discuss the principles of how organisms can adapt or respond to their environment
  • Translate knowledge on genetics and omics into breeding strategies
  • Use online genomics resources
  • Design an ecogenomics approach to answer a biological research question
  • Carry out an ecogenomics research project
  • Analyse the results of a Genome Wide Association Study
  • Interpret the practical results
  • Write a scientific paper about the practical results

Teaching methods

  • Lecture
  • Computer lab session/practical training
  • Fieldwork/excursion
  • Self-study
  • Working independently on e.g. a project or thesis
  • Supervision/feedback meeting
  • Presentation/symposium

Learning activities

Activiteit

Aantal uur

Computerpracticum

43

Excursie

8

Hoorcollege

30

Practicum

71

Tentamen

3

Vragenuur

2

Werkcollege

20

Zelfstudie

150

Academic skills

In the course you will learn how omics and data analysis tools and mapping can be used to unravel mechanisms underlying the interaction of organisms with their environment; you will use these techniques to design an ecogenomics experiment, execute that experiment, analyse the data, and create a report in which you analyse and interpret these data.

Attendance

Programme's requirements concerning attendance (OER-B):

  • Participation in all practical (computer) sessions, field work and seminars in the curriculum is in principle obligatory. Any additional requirements are described per section in the course manual. Also the possible consequences of not fulfilling this obligation are described.

Additional requirements for this course:

Attendance at lectures is highly recommended. Attendance during practical components is mandatory. The latter also applies to all (computer) practicals and tutorials. If you are unable to participate due to personal circumstances (such as illness or special family circumstances), please contact the course coordinator by e-mail. The course coordinator will then discuss with you whether there are options to participate/contribute in an alternative way (e.g. online) or compensate your absence later. If you are not able to follow education for a long time (longer than 1 week), please also contact the study advisor.

NB Covid-19: Stick to the RIVM guidelines at all times, even if this means that you have to miss one or more mandatory classes. In this case too, please contact the course coordinator directly so that a solution can be found together.

Assessment

Item and weight Details

Final grade

0.5 (50%)

Tentamen

Must be ≥ 5

0.4 (40%)

Practical

Mandatory

0.1 (10%)

Journal club

Mandatory

Final grade after retake

0.5 (50%)

Hertentamen

Must be ≥ 5

0.4 (40%)

Practical

0.1 (10%)

Journal club

The final grade is composed of the exam (0.5), the practical (report, presentation, attitude)(0.4) and the journal club (0.1). A student has passed the course when the exam scores 5.0 or higher and the weighted final grade is 5.5 or higher. If the practical report is submitted after the deadline without a good reason the maximum grade that can be obtained is 6.0. If the practical report is of insufficient quality the student will be given the opportunity to improve it; the maximum grade that can then be obtained is 6.0.

Inspection of assessed work

For at least twenty working days after the announcement of the results of a written examination, the student can, on request, inspect his/her assessed work, the questions and assignments set, as well as the standards applied for marking. The place and time will be announced via Blackboard (from Teaching and Examination Regulations).

Assignments

Presentation

  • Presentation about research papers with a group of students, individually graded

Oral presentations

  • Oral presentations about practical results

Written report

  • Written report of practical work

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

Weeknummer Onderwerpen Studiestof
1    
2 Formative assessment See Canvas
3 Formative assessment See Canvas
4 Formative assessment See Canvas
5    
6    
7    
8 Practical report  

Timetable

The schedule for this course is published on DataNose.

Additional information

The course is meant for 3rd year Biology students. Content of the 2nd year UvA Biology courses will be considered as known. Students from outside the UvA, who did not follow these courses, should contact the course coordinator before registration.

Maximum 30 students

Processed course evaluations

We have introduced formative assessment and make available a practice exam to prepare the students for the exam. We have introduced a tutorial on statistical analysis to support the students in their data analysis. We have introduced extra classes/tutorials on GWAS to strengthen the background of the students on that topic. The Canvas  page  of the course has been restructured and the learning objectives reformulated. 

Contact information

Coordinator

  • prof. dr. ir. Harro Bouwmeester

Staff

  • dr. Thomas Blankers
  • dr. P.M. Bleeker
  • dr. L. Fokkens
  • dr. ir. P.F. Fransz
  • prof. dr. A.T. Groot
  • dr. rer. nat. Anna Heintz Buschart
  • S.W. Alves Aflitos-Hoogstrate MSc
  • dr. M. Kant
  • P. Kuperus
  • prof.dr. M.E. Schranz
  • dr. ir. R.C. Schuurink
  • Hui Sheng