Separation Sciences

6 EC

Semester 1, period 1

52548SES6Y

Owner Master Chemistry (joint degree)
Coordinator prof. dr. Govert Somsen
Part of Master Chemistry (joint degree), track Analytical Sciences,

Course manual 2019/2020

Course content

The course comprises theory and practice of liquid and gas chromatography, and of capillary electrophoresis, including the common detection principles used with these separation techniques.

The fundamental principles of chromatography and capillary electrophoresis (e.g. retention, migration, dispersion, stationary-phase properties) and signal processing (e.g. statistical moments) will be addressed. The characteristics and differences of packed-column and open-tubular separations will be outlined. Attention will be paid to instrumental aspects, such as injection systems for gas chromatography.

For liquid chromatography, various phase systems including normal-phase, reversed-phase, and ion-exchange, will be discussed. The principles and application of gradient elution will be treated.

Other topics include an introduction to two-dimensional liquid and gas chromatography, sample preparation, and polymer separations.

As part of the course, students will give a short presention on a scientific paper on separations.

 

Study materials

Literature

  • will be available on Canvas

Other

  • Handouts of the lectures

    Exercises for problem solving

Objectives

  • Understand and explain the fundamentals of different separation methods;
  • Use different performance parameters to compare methods;
  • Predict the effects of experimental parameters on the performance of methods and use this for optimizaton;
  • Translate experimental data into chemical information;
  • Propose suitable methods for various applications;
  • Read, summarize and present relevant scientific papers.

Teaching methods

  • Lecture
  • Seminar
  • Presentation/symposium

Learning activities

Activity

Number of hours

Lectures/seminars

44

Presentation incl. preparation

12

Zelfstudie

120

 

Attendance

The programme does not have requirements concerning attendance (OER-B).

Additional requirements for this course:

The literature presentation is a requirement. Without literature presentation no grade will be provided.

Assessment

Item and weight Details

Final grade

1 (100%)

Tentamen 1

The grade for the written exam should be at least 5.0.

Inspection of assessed work

Contact the course coordinator to make an appointment for inspection.

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

wk day date room lecturer topic    
               
36 Tue 3-Sep see DataNose or schedule on Canvas BP Fundamentals of Chromatography; Dispersion
  Fri 6-Sep   BP Peaks and Moments  
37 Tue 10-Sep   GS LC modes  
  Fri 13-Sep   GS CE  
38 Tue 17-Sep   BP Gradient Elution
  Fri 20-Sep   BP GC1  
39 Tue 24-Sep   GS Sample preparation
  Fri 27-Sep     Lectures @ WGS meeting
40 Tue 1-Oct   PS GC 2
  Fri 4-Oct   PS GCxGC
41 Tue 8-Oct   GS+BP Detection + LCxLC
  Fri 11-Oct   PS Polymer Separation   QA hour
42 Tue 15-Oct   --- Student presentations
  Fri 18-Oct   --- Student presentations
               
43 Tue 22-Oct   -- Exam

Contact information

Coordinator

  • prof. dr. Govert Somsen

Docenten

Dr. Bob Pirok (BP)

Prof. Peter Schoenmakers (PS)

Prof. Govert Somsen (GS)