Mass Spectrometry

6 EC

Semester 1, period 2

5254MASP6Y

Owner Master Chemistry (joint degree)
Coordinator dr. Steen Ingemann Jorgensen
Part of Master Chemistry (joint degree), track Analytical Sciences, year 1

Course manual 2017/2018

Course content

The course covers basic principles of mass spectrometry (MS) and the application of the method in chemistry and life sciences. The following topics are treated:

  • The principles of the Ionization methods: Electron Ionisation (EI), Chemical Ionisation (CI), Electrospray Ionisation (ESI) and Matrix Assisted Laser Desorption Ionisation (MALDI).
  • Basic principle and analytical features of common mass spectrometers, such as Sector, Time-of-flight, quadrupoles and Ion trap instruments. 
  • Structure determination of (bio)organic compounds based on dissociation reactions of ions in the gas phase, interpretation of mass spectra and MS-MS analysis.
  • (Bio)analytical Mass Spectrometry and in particular the role of hyphenated methods, GC-MS, LC-MS and LC-MS-MS in (bio)chemical analysis and drug development and quantitative analysis of (bio)organic compounds.
  • Proteomics covering the application of mass spectrometry in identification of proteins, peptide mass fingerprinting, sequence tag analysis, analysis of post translational modifications quantitative proteomics, interpretation of peptide MS/MS spectra.

Study materials

Literature

  • Edmond de Hoffmann, Jean Charette and Vincent Stroobant, 'Mass Spectrometry Principles and Applications', John Wiley & Sons and lecture notes.

Syllabus

  • Basic Principles of Mass Spectrometry

Objectives

  • Know the basic principles of common mass spectrometric Instrumentation;
  • Know the principle of common ionisation methods;
  • Know the common dissociation reactions of molecular ions and protonated molecules;
  • Be able to interpret mass spectra of simple (bio)organic compounds;
  • Know the combinations between chromatographic methods and mass spectrometry;
  • Be able to devise a procedure for quantitative analysis of compounds with the use of the combination of a chromatographic method and mass spectrometry;
  • Know the basics of the application of mass spectrometry in proteomics.

Teaching methods

  • Lecture
  • Self-study
  • Seminar

Lectures and problem solving sessions are integrated.

Learning activities

Activity

Number of hours

Hoorcollege

28

Tentamen

3

Werkcollege

21

Zelfstudie

116

Attendance

The programme does not have requirements concerning attendance (OER-B).

Assessment

Item and weight Details

Final grade

1 (100%)

Tentamen

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.uva.nl/plagiarism

Timetable

The schedule for this course is published on DataNose.

Additional information

Recommended prior knowledge: organic chemistry, biochemistry and physics at the level of a BSc Chemistry.

Contact information

Coordinator

  • dr. Steen Ingemann Jorgensen