Immunologie Research en Kliniek

Immunology Research and Clinic

Studiewijzer 2025/2026

Globale inhoud

The lectures:

• Interactions between immunological systems (innate-acquired/immunological cells and soluble immunological factors)
• Cell biological processes in immunology (synapse formation, signal transduction, antigen presentation)
• Antibodies and autoimmunity (IgG4 versus IgG1, immune complexes, RA, therapeutic antibodies, B cell differentiation)
• Inflammation and complement activation (cell death and inflammation, complement regulation)
• Tumor immunology (dendritic cell therapy, adoptive T cell transfers, TLR agonists)
• Big data analyses in immunology

The seminars:

• FACS assignment based on the FACS-diva program
• The structure of proteins using the proximity plot
• Statistical assignment

The experiments:

• Purification of granulocytes followed by a phagocytosis experiment.
• Purification of monocytes by density separation followed by positive selection with antibody coupled magnetic beads.
• The differentiation of immature dendritic cells from the purified monocytes.
• Maturation of immature dendritic cells by cytokine cocktails or TLR-agonists.
• Measuring different cytokines with ELISA.
• Purity control of monocytes with FACS.
• Determination of the activity of the classical and the alternative complement route in patient sera using hemolytic tests.
• Creating and presentation of a research proposal based on techniques and knowledge obtained in this course.

Studiemateriaal

Literatuur

• Janeway's Immunobiology, 10th edition, Kenneth M. Murphy ISBN 9780393884913

Practicummateriaal

• Laboratory coat is mandatory.

• Laboratory notebook is mandatory.

Software

• LabBuddy (Link on Canvas)

• FACS Diva (Available on UvA laptops)

• Script.R (Link on Canvas)

Leerdoelen

• The student can explain the theory of main immunological interactions and cell biological process in immunology and discuss about their implications.
• The student can explain the theory of main processes and therapies in tumor immunology and discuss about their implications.
• The student can explain the role of antibodies in autoimmunity and discuss about its implications.
• The student can explain the processes involved in inflammation and discuss about its implications.
• The student can explain how big data in immunology can be analysed.
• The student is able to apply his or her own knowledge to solve structure-functional questions in immunology in computer practical.
• The student is able to independently perform commonly used immunological techniques.
• The student is able to draw conclusions of the results obtained from the immunological techniques and substantiate them.
• The student is able to succinctly report the data obtained from the immunological techniques in a lab journal.
• The student can use relevant software and bioinformatics tools to analyze immunological data.
• Under the supervision of a researcher, the student is able to formulate an immunological research proposal based on scientific literature.
• The student is able to present a research proposal in English to fellow students in a clear manner.

Onderwijsvormen

• Werkcollege
• Zelfstudie
• (Computer)practicum
• Hoorcollege
• Veldwerk/excursie
• Presentatie/symposium
• Zelfstandig werken aan bijv. project/scriptie

Bijdrage aan het leerproces per onderwijsvorm:

Hoorcolleges

• Verdieping van kennis over immunologie.

Practicumlessen / Zelf studie

• Ontwikkeling van technische vaardigheden in immunologische laboratoriumtechnieken door het uitvoeren van diverse experimenten.
• Stimuleren van academische en praktische vaardigheden.
• Training van documentatievaardigheden door het bijhouden van een labjournaal.
• Zelfstandig voorbereidingen inplannen.

Laptopcolleges

• Vaardigheden in het analyseren van data.

Werkcolleges

• Ontwikkeling van ondersteunende vaardigheden, zoals statistiek.

Zelfstandig en in groepsverband werken aan project / Presenteren

• Ontwikkeling van diverse vaardigheden, waaronder verbanden leggen tussen theorie en practicum, professionele vaardigheden en het presenteren van onderzoeksplan.

Excursie

• Inzicht geven in mogelijkheden binnen de research.

Deze onderwijsvormen dragen bij aan een breed scala aan kennis en vaardigheden, essentieel voor het leerproces.

Verdeling leeractiviteiten

Aanwezigheid

Aanvullende eisen voor dit vak:

• Participation in all practicals, computer practicals, presentation, excursion and seminars in the curriculum is mandatory.
• Up to two practicals may be missed. In case of absence, the practicals must be attended at another time or a replacement task must be completed.
• Presence at the lectures is strongly recommended.
• All practicals and seminars are compulsory. If you are unable to attend for one day, e.g. in case of illness, we will search for a solution together with you.
• Please report your absence to c.g.m.kuijk@uva.nl
• If you have not been present please contact us to determine how to catch up on the missed practical.
• Make sure you own the missed lesson material on the day you come back. On this day you must also have prepared the practical.
• If you are not able to follow an education for a long period of time (longer than 1 week), please contact the study advisor.

Toetsing

• A student has passed the course if the final grade is 5.5 or higher. Both the theoretical and practical components must be rated at least 5. In addition, it is mandatory that the partial test of the practical is also assessed with at least a 5.
• There is a resit option for both the exam and the partial test of the practical.
• If the student obtains a pass for the practical, but an insufficient score for the theory, the assessment of the practical remains valid for one year.
• Students who pass the theory but fail the practical cannot resit the practical until the following academic year. In this case, the theory grade also remains valid for one year.

Inzage toetsing

Om een inzagemoment aan te vragen, kun je contact opnemen met de coördinator.

As soon as the (partial) figures are known, they can be viewed in consultation.

Opdrachten

Research Project

• The students will present a research plan in collaboration with a PhD student

The assignment is carried out in groups of 3. The feedback is displayed using a Rubic.

Fraude en plagiaat

Dit vak hanteert de algemene 'Fraude- en plagiaatregeling' van de UvA. Hier wordt nauwkeurig op gecontroleerd. Bij verdenking van fraude of plagiaat wordt de examencommissie van de opleiding ingeschakeld. Zie de Fraude- en plagiaatregeling van de UvA: http://student.uva.nl

Weekplanning

Aanvullende informatie

This course has a CANVAS site. Here you will find the necessary additional information, such as the group division of the seminars, the assignments and the schedule. So check the CANVAS site regularly!

Vaccination against hepatitis B is mandatory. If you have any questions, please contact: HepBvaccinatie-science@uva.nl

Verwerking feedback studenten

The excursion has been rescheduled at the end of the course.

Contactinformatie

Coördinator

• prof. dr. S.M. van Ham

Docenten

• Ing. Carlijn Kuijk
• Ing. Irma Rensink
• Prof. dr. Marieke van Ham
• Dr. Anja ten Brinke
• Dr. Sabrina Pollastro
• Dr. Gertjan Wolbink
• Dr. Theo Rispens
• Dr. Jaap van Buul
• Dr. Richard Pouw