Engineering Mammalian Systems

Studiewijzer 2025/2026

Globale inhoud

Biomedical researchers have long used animal species or simplified in vitro cellular systems to understand and develop treatments for a range of human diseases. Although there have been some notable successes, the truth is that much of the ‘translational’ research acquired in model systems, has failed to generalize to humans. This so-called “Translatability Crisis” recognizes declining research efficiency; whereby global spending on research increases, yet ‘effective drug’ output stagnates.

One part of bridging this gap, is to develop better model systems, such that the basic discoveries align with clinical reality. We are at an important moment in science where researchers recognize the need for advanced human-based model systems. As a result, current technological innovation is focusing on developing human-based in vitro (lab-grown) models. These models aim to speed up experimental discoveries and the delivery of new treatments to patients.

This course will explore this topic, through a variety of perspectives – historical, ethical, practical and critical. We aim to provide you with the context, concepts, and skills needed to not only to critically evaluate these emerging technologies, but hopefully to inspire you to imagine the future possibilities that could shape the next wave of innovation in biomedical research.

Equipping you to assess today’s breakthroughs, and envision tomorrow’s innovations.

Studiemateriaal

Literatuur

• Lecture (Hoorcollege) Powerpoints

Leerdoelen

• Recall the key terminology and general principles/components of various advanced in vitro models (organoids, organ-on-a-chip, iPSCs) and their supporting technology (microfluidics, mechanobiology).
• Explain the biological rationale behind using various advanced in vitro models to study mammalian physiology and disease, in terms of structure, complexity, and relevance.
• Compare and contrast various advanced in vitro models in terms of their suitability for specific research questions.
• Interpret experimental data from advanced in vitro systems to identify trends, anomalies, and potential sources of error.
• Critically assess the ethical, practical, and scientific limitations of advanced mammalian in vitro models, as compared to each other and to in vivo (animal) systems.

Onderwijsvormen

• Hoorcollege
• Werkcollege
• Laptopcollege
• Presentatie/symposium
• Zelfstudie

This course examines the biological and technological foundations of human and animal models in the biomedical sciences and related industries. Students will trace the historical development of animal models, progress through methods for culturing mammalian cells and tissues, and finally engage with state-of-the-art approaches for creating three-dimensional cellular systems such as organoids, gastruloids, and organ-on-a-chip platforms. Particular focus will be placed on innovative technologies designed to reduce reliance on animal testing. These include microfluidic and organ-on-a-chip systems, where students will explore how design principles, fluid dynamics and substrate properties shape cell migration, differentiation, and function.

The theoretical content is integrated with practical training: through computer-based sessions, students will gain hands-on experience with experimental data from model systems, taking the chance to analyze, evaluate and critique their use tp reinforce understanding of experimental design and application. Complementing this, the tutorial series provides a forum for critique, debate, and creative problem-solving, enabling students to engage more deeply with the opportunities and challenges of advanced model systems. Together, these components lay the groundwork for applying and innovating in vitro technologies in both academic and industrial biomedical research.

Verdeling leeractiviteiten

Aanwezigheid

Aanvullende eisen voor dit vak:

Attendance at the tutorials and computer practicals are mandatory as they are directly linked (and test) one or more of the course objectives. Tutorials: ILO#2, ILO#3, ILO#5. Computer Practicals: ILO#4

Absence needs to be communicated in advance to the course coordinator.

Toetsing

The final exam (digital, 60% final grade) will test the theory of the lecture series content (#ILO1) in multiple choice and short answer question format. In addition, the exam will contain example scenarios in which the students must logically rationalize and explain model choice and/or critique, as practiced in the tutorial series (ILO#2, ILO#5). No supporting information may be permitted into the exam. A resit exam will be made available for those who fail to score above 5.

The assignments, each contribute towards the final grade. Adequate participation and completion of the WC model design WCs (pass/fail), and the WC symposium/presentations (10% final grade), and finally submission of the computer practical assignment (10% final grade). 

Fraude en plagiaat

Dit vak hanteert de algemene 'Fraude- en plagiaatregeling' van de UvA. Hier wordt nauwkeurig op gecontroleerd. Bij verdenking van fraude of plagiaat wordt de examencommissie van de opleiding ingeschakeld. Zie de Fraude- en plagiaatregeling van de UvA: http://student.uva.nl

Weekplanning

Aanvullende informatie

Language

This course will be offered in English.

Use of AI

Throughout the course, students are expected to research various aspects and gather important information themselves (for assignments and tutorials). In the current climate, information retrieval includes the use of AI models – and therefore in this course, students are free to make choices themselves regarding which source(s) they use and how. We expect students to use appropriate critique when retrieving information, and be wary of the limitations of such systems. Your grade will be yours – so don’t blindly trust everything you read – check original sources and use your scientific judgement!

Contactinformatie

Coördinator

• dr. Caitrín Crudden PhD

c.j.crudden@uva.nl