Advanced Separation Sciences

Course manual 2025/2026

Course content

In this course a number of separation methods will be discussed more in depth compared to the compulsory Separation Sciences course. Techniques discussed will be:

• electrophoretic methods;

• field flow fractionaton

• specialised LC separation modes like mixed mode chromatography, chiral separation

• multidimensional liquid chromatography

Study materials

Literature

• Will be made available on Canvas

Syllabus

• Will be made available on Canvas

Other

• Hand-outs, tutorials and review articles. The book Analytical Separation Science can be used as support to the learning. https://doi.org/10.1039/9781837674824

Objectives

• Comprehend the scientific principles underlying the following separation techniques: specialized liquid separation modes, capillary electrophoresis, field-flow fractionation techniques and multidimensional chromatography.
• Be able to discuss similarities and differences between learned separation techniques.
• Be able to apply this knowledge to determine the key experimental parameters required to optimise the aforementioned separation techniques.
• Be able to develop methods for real-life applications suitable to each separation technique through guided tutorials.

Teaching methods

• Lecture
• Self-study
• Seminar

Lectures, tutorials and lab tours (to discuss practical aspects on learned techniques). 

Learning activities

Attendance

This programme does not have requirements concerning attendance (TER part B).

Additional requirements for this course:

Absence needs to be communicated to the course coordinator.

Assessment

The Separation Science Challenge assignment is worth 20% of the course grade

Assignments

Separation Science Challenge

• Group work to prepare a presentation on a scientific topic on the basis of the topic discussed in the course

The Separation Science Challenge assignment is worth 20% of the course grade

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

Additional information

Prior knowledge: students should have taken the course 'Separation Sciences', or a similar course.

Contact information

Coordinator

• dr. A. Gargano

Staff

• dr. B.W.J. Pirok
• dr. K. Joss
• dr. Alina Astefanei
• dr. Andrea Gargano