Advanced Spectroscopy

Course manual 2024/2025

Course content

The topics discussed include modern applications of fluorescence/phosphorescence and Raman spectroscopy. Attention will be given to energy transfer mechanisms and the use of fluorescent probes, high-resolution fluorescence at cryogenic temperatures, single-molecule spectroscopy and coupling to analytical separation techniques. Raman spectroscopic topics will include surface-enhanced Raman, resonance Raman, time-resolved Raman and non-linear Raman techniques. Instrumental aspects, such as laser excitation, time-resolved detection, polarization and imaging will also be covered in this course.

Recent examples of the use of these techniques in a chemistry, medical, environmental, industrial, forensic or space research context will be discussed on the basis of literature presentations by the students. The course also includes a set of fluorescence and Raman experiments at VU LaserLaB. In small groups the students will carry out a set of experiments, of which the results will be laid down in a report and an oral presentation.

The students will also prepare a presentation on a recent literature article, to be given and discussed in class.

Study materials

Practical training material

• Literature, lecture handouts and instructions for the experiments will be made available through Blackboard.

Objectives

• To acquire a deeper insight into the basic principles and modern developments of molecular spectroscopy in (bio)analytical chemistry, with emphasis on fluorescence/luminescence and Raman techniques.
• To become familiar with recent literature on the use of these techniques in a variety of applications.
• To improve written and oral presentation skills.
• To acquire practical skills in modern (laser) spectroscopy.

Teaching methods

• Lecture
• Computer lab session/practical training
• Presentation/symposium
• Supervision/feedback meeting
• Working independently on e.g. a project or thesis

Learning activities

Attendance

This programme does not have requirements concerning attendance (TER part B).

Assessment

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

Additional information

Recommended prior knowledge: Background knowledge of molecular spectroscopic techniques, in particular fluorescence and Raman, is expected (e.g. MSc Chemistry courses (Bio)Molecular spectroscopy and Microscopy and Spectroscopy).

Contact information

Coordinator

• Freek Ariese