Analytical NMR spectroscopy

6 EC

Semester 2, period 4

5254ANNS6Y

Owner Master Chemistry (joint degree)
Coordinator dr. T.A. Hamlin
Part of Master Chemistry (joint degree), track Analytical Sciences, Master Chemistry (joint degree), track Chemistry of Life, year 1

Course manual 2023/2024

Course content

This course deals will cover the concepts of chemical shift, spin-spin coupling, experimental NMR setup, symmetry and chirality, 1H, 13C, and other heteronuclear NMR, and 2D NMR. NMR pulse sequences will also be discussed at length. Analytical applications of NMR will also be discussed, including the use of NMR for quantification of small molecules, NMR-based metabolomics, and the way NMR can be coupled to other analytical techniques in hyphenated set-ups like liquid chromatography-MS/NMR.

Study materials

Literature

  • Günther, ‘NMR Spectroscopy: Basic Principles, Concepts and Applications in Chemistry’ Third Edition, Wiley-VCH.

Syllabus

  • Please see Canvas.

Practical training material

  • Book problems, handouts, and supporting material.

Objectives

  • Acquire a physical understanding of the concepts of one- and two-dimensional NMR techniques.
  • Understand the role and application of NMR in the different fields of organic and analytical chemistry.
  • Analyze and interpret both 1D (1H, 13C, and selected heteronuclear) and 2D spectra of molecules.
  • Understand the role and application of NMR to real-life analytical chemistry applications (e.g. quantitative NMR, metabolomics, and NMR hyphenation: LC-NMR/MS)

Teaching methods

  • Lecture
  • Seminar
  • Exercises
  • Fieldwork/excursion
  • Fieldwork/excursion

Learning activities

Activity

Number of hours

Lectures

13.5

Seminar/tutorials

18
Written assignment

 

8
Excursion

4

 

Self-study

rest

 

Attendance

This programme does not have requirements concerning attendance (TER part B).

Assessment

Item and weight Details

Final grade

1 (100%)

Tentamen 1

The exam covers all content covered during the entire course.

Inspection of assessed work

Contact the course coordinator to make an appointment for inspection.

Fraud and plagiarism

The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl

Course structure

Weeknummer Onderwerpen Studiestof
1 Introduction; chemical shift; spin-spin coupling Ch.1-3
2 Instrumentation; proton NMR Ch. 4-5
3 Symmetry and chirality; computational NMR Ch. 7
4 2D NMR Ch. 9
5 13C NMR, experimental NMR lab Ch. 11
6

Quantitative NMR, NMR-based Metabolomics

  
7

NMR hyphenation: LC-NMR/MS

  
8 Exam  

Additional information

Please see the canvas page for course management and additional details.

Contact information

Coordinator

  • dr. T.A. Hamlin