6 EC
Semester 1, period 1
52548SES6Y
| Owner | Master Chemistry (joint degree) |
| Coordinator | prof. dr. Govert Somsen |
| Part of | Master Chemistry (joint degree), track Analytical Sciences, Master Forensic Science, year 2 |
The course comprises theory and practice of liquid and gas chromatography, and of capillary electrophoresis, including the common detection principles used with these separation techniques.
The fundamental principles of chromatography and capillary electrophoresis (e.g. retention, migration, dispersion, stationary-phase properties) and signal processing (e.g. statistical moments) will be addressed. The characteristics and differences of packed-column and open-tubular separations will be outlined. Attention will be paid to instrumental aspects, such as injection systems for gas chromatography.
For liquid chromatography, various phase systems (modes) including normal-phase, reversed-phase, and ion-exchange, will be discussed. The principles and application of gradient elution will be treated.
Other topics include an introduction to two-dimensional gas chromatography, sample preparation, and polymer separations.
As part of the course, students will give a short presentation on a scientific paper on separations.
will be available on Canvas
Handouts of the lectures
Exercises for problem solving
|
Activity |
Number of hours |
|
Lectures/seminars |
56 |
|
Presentation incl. preparation |
12 |
|
Self study |
60 |
This programme does not have requirements concerning attendance (TER part B).
Additional requirements for this course:
The literature presentation is a requirement. Without literature presentation no grade will be provided.
| Item and weight | Details |
|
Final grade | |
|
0.8 (100%) Written exam | Must be ≥ 5 |
- The written exam, short quizzes and presentation are graded.
- The grade of the written exam (E), average grade of the short quizzes (Q), and grade for the presentation (P) result in the final grade which equals 0.8*E + 0.1*Q + 0.1*P.
- If Q < E than final grade equals 0.9*E + 0.1*P.
- The grade for the written exam should be at least 5.0.
Contact the course coordinator to make an appointment for inspection.
Pairs of students provide a short presentation on a scientific paper on separations. The presentation will be graded.
The 'Regulations governing fraud and plagiarism for UvA students' applies to this course. This will be monitored carefully. Upon suspicion of fraud or plagiarism the Examinations Board of the programme will be informed. For the 'Regulations governing fraud and plagiarism for UvA students' see: www.student.uva.nl
| wk | day | date | room | lecturer | topic | ||
| 36 | Tue | 5-Sep | see DataNose or time schedule on Canvas | GS&BP | Intro to course and chromatography | ||
| Wed | 6-sep | BP | Chromatographic parameters | ||||
| Fri | 8-Sep | BP | Dispersion | ||||
| 37 | Tue | 12-Sep | GS | LC modes I | |||
| Wed | 13-Sep | Short quiz I | |||||
| Fri | 15-Sep | BP | Peaks & moments | ||||
| 38 | Tue | 19-Sep | GS | LC Modes II | |||
| Wed | 20-Sep | BP | Gradients | ||||
| Fri | 22-Sep | GS | Sample Preparation | ||||
| 39 | Tue | 26-Sep | GS | LC detection | |||
| Wed | 27-Sep | Short quiz 2 | |||||
| Fri | 29-Sep | BP | Polymer Separations | ||||
| 40 | Tue | 3-Oct | GS | CE I | |||
| Wed | 4-Oct | GS | CE II | ||||
| Fri | 6-Oct | GS | CE detection/injection & MEKC | ||||
| 41 | Tue | 10-Oct | PS | Open tubular chromatography | |||
| Wed | 11-Oct | PS | GC & injection; Short quiz 3 | ||||
| Fri | 13-Oct | PS | 2D GC | ||||
| 42 | Tue | 17-Oct | Student presentations | ||||
| Wed | 18-Oct | BP/GS/PS | Question hour | ||||
| Fri | 20-Oct | Student presentations | |||||
| 43 | Tue | 24-Oct | Exam | ||||
The schedule for this course is published on DataNose.
Dr. Bob Pirok (BP)
Prof. Peter Schoenmakers (PS)
Prof. Govert Somsen (GS)